Newer approach of screening test for antinuclear antibodies: An enzyme-linked immunosorbent assay detecting antinuclear antibodies characteristic of connective tissue diseases.
نویسندگان
چکیده
منابع مشابه
Identification of specific antinuclear antibodies in dogs using a line immunoassay and enzyme-linked immunosorbent assay.
Circulating antinuclear antibodies (ANA) are commonly present in the systemic autoimmune disease Systemic Lupus Erythematosus (SLE) and in other systemic rheumatic diseases, in humans as well as in dogs. The indirect immunofluorescence (IIF)-ANA test is the standard method for detecting ANA. Further testing for specific ANA with immunoblot techniques or ELISAs is routinely performed in humans t...
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The objective of this study was to compare an indirect ELISA, based on a purified 60 kDa envelope glycoprotein (gp51SU), with a Pourquire indirect ELISA for the detection of antibodies to the bovine leukemia virus. For conducting this research, 340 serum samples were collected from two different breeds of cows (Sarabi and Holestin) in different herds. Commercial ELISA revealed positive results ...
متن کاملAntinuclear Antibodies
knowledge of gene regulation, particularly in neoplastic cells, this deficit is striking. With our current understanding of chromosomal translocations, point mutations, and gene amplification in tumor cells, it is surprising to read that there is ". . . no difference between cancer and normal DNA . . ." (p. 130). The author spends a fair amount of time discussing possible mechanisms for the car...
متن کاملAntinuclear antibodies and their detection methods in diagnosis of connective tissue diseases: a journey revisited
It has been more than 50 years since antinuclear antibodies were first discovered and found to be associated with connective tissue diseases. Since then different methods have been described and used for their detection or confirmation. For many decades immunofluorescent antinuclear antibody test has been the "gold standard" in the diagnosis of these disorders. However to increase the sensitivi...
متن کاملThe analysis of specific antinuclear antibodies concentrations in children with connective tissue diseases
Methods We observed 187 children from 2 to 18 years (median 13 years, 25‰ – 9 years, 75‰ – 15 years); 70 (37,4%) boys, 117 (62,6%) – girls with systemic lupus erythematosis (SLE), juvenile rheumatoid arthritis (JRA), spondyloarthritis (SA), scleroderma (systemic/localized), dermatomyosites and mixed CTD (MCTD). All patients undergo common rheumatologic examination. Specific ANA were measured wi...
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ژورنال
عنوان ژورنال: Japanese Journal of Clinical Immunology
سال: 1997
ISSN: 0911-4300,1349-7413
DOI: 10.2177/jsci.20.417